マイナスからプラスを生み出す環境負価低減バイオプロセスの開発

発表論文・著書リスト

〇総説

・Hara KY, Kobayashi J, Yamada R, Sasaki D, Kuriya Y, Hirono-Hara Y, Ishii J, Araki M, Kondo A. Transporter engineering in biomass utilization by yeast. FEMS Yeast Res. 2017 Nov 1;17(7). doi: 10.1093/femsyr/fox061. PubMed PMID: 28934416.

・Hara KY, Kondo A. ATP regulation in bioproduction. Microb Cell Fact. 2015 Dec 10;14(1):198. PubMed PMID: 26655598. Free PMC article

・Kiyotaka Y. Hara, Michihiro Araki, Naoko Okai, Satoshi Wakai, Tomohisa Hasunuma, Akihiko Kondo (2014) Development of bio-based fine chemical production through synthetic bioengineering. Microbial Cell Factries 13(1), 173. Free PMC article

・Tomohisa Hasunuma, Fumiyoshi Okazaki, Naoko Okai, Kiyotaka Y. Hara, Jun Ishii, Akihiko Kondo (2013) A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology. Bioresourse Technology 135, 513-522.

・Akihiko Kondo, Jun Ishii, Kiyotaka Y. Hara, Tomohisa Hasunuma, Fumio Matsuda (2013) Development of microbial cell factories for bio-refinery through synthetic bioengineering. Journal of Biotechnololgy 163(2), 204-216.

〇著書

・原清敬　(2023) 「食品ロス削減と食品廃棄物資源化の技術」　第13章　微生物を用いた飲料抽出残渣からのファインケミカルの発酵生産技術の開発（CMC出版）

・原清敬　(2021)「シリョク,キオク,シンパクを光でつなぐタンパク」生物工学会誌　バイオミディア（日本生物工学会）99(5), 252（単著）記事公開

・Kobayashi, J., Sasaki, D., Hara, K.Y., Kondo, A.(2018) Genetic engineering of microorganisms for high glutathione production, Glutathione: Biosynthesis, Functions and Biological Implications, Nova Science Publishers, in press

・原清敬　(2016)「共生由来オルガネラの改変―共生が駆動する微生物発酵生産」生物の科学　遺伝（2016年3月号）特集　真核細胞の共生由来オルガネラ研究最前線―広がり続ける多様性と機能（NTS出版）70, 110-114 記事公開

・原清敬、近藤昭彦　(2014) 28章バイオマス利用技術化学便覧、日本化学会編、応用化学編第7版（丸善出版）1615-1651（分担執筆）28.5.3節担当

・Ye Xiaoting、原清敬　(2014)「もっと光を！人工光合成細菌の魅力」生物工学会誌　バイオミディア（日本生物工学会）92, 444（共著）

・原清敬、下立夏香、森英郎 (2010) 「大腸菌ATP供給活性向上変異体の効率的探索と物質生産への応用」、 バイオサイエンスとインダストリー（バイオインダストリー協会）68,190-193（共著）

・Kiyotaka Y. Hara (2009) Methods in Molecular Biology: Methods and Protocols, Reverse Chemical Genetics (Section 2.7. Permeable Cell Assay: a method for high-throughput measurement of cellular ATP synthetic activity), Methods in Molecular Biology (Humana Press) 577, 251-257（分担執筆）

・原清敬、吉田賢右 (2001)「モチーフドメインリスト」生体の科学（医学書院）52, 502-503（分担執筆）

〇原著論文

・Daicho KM, Hirono-Hara Y, Kikukawa H, Tamura K, Hara KY: Engineering yeast with a light-driven proton pump system in the vacuolar membrane. Microbial Cell Factories, 2024 Jan; 23:4. doi: 10.1186/s12934-023-02273-1.Free PMC article

・Kikukawa H, Ando A, Hannya A, Asras MFF, Okuda T, Sakamoto T, Hara KY, Sakuradani E, Ogawa J: Mead acid production by disruption of Δ12-desaturase gene in Mortierella alpina 1S-4. J Biosci Bioeng. 2023 Nov;136(5):353-357. doi: 10.1016/j.jbiosc.2023.08.001.

・Kikukawa H, Nagao T, Ota M, Takashima S, Kitaguchi K, Yanase E, Maeda S, Hara KY: Production of a selective antibacterial fatty acid against Staphylococcus aureus by Bifidobacterium strains. Microbiome Research Reports; 2023 Feb 22; 2:4. Free PMC article

・Sano M, Tanaka R, Kamata K, Hirono-Hara Y, Ishii J, Matsuda F, Hara KY, Shimizu H, Toya Y: Conversion of Mevalonate to Isoprenol Using Light Energy in Escherichia coli without Consuming Sugars for ATP Supply. ACS Synth Biol. 2022 Dec 16;11(12):3966-3972. doi: 10.1021/acssynbio.2c00313.

・Otsuka K, Seike T, Toya Y, Ishii J, Hirono-Hara Y, Hara KY, Matsuda F: Evolutionary approach for improved proton pumping activity of heterologous rhodopsin expressed in Escherichia coli. J Biosci Bioeng. 2022 Dec;134(6):484-490. doi: 10.1016/j.jbiosc.2022.08.006.

・Hirono-Hara Y, Miyuu Y, Hara KY: Active transglutaminase production from synthetic whey using engineered Saccharomyces cerevisiae. Bioresource Technology Reports;2022 September; 19:101154 https://doi.org/10.1016/j.biteb.2022.101154

・Kobayashi J, Sasaki D, Hara KY, Hasunuma T, Kondo A：A Metabolic engineering of the L-serine biosynthetic pathway improves glutathione production in Saccharomyces cerevisiae. Microbial Cell Factories. 2022 Aug 6;21(1):153. doi: 10.1186/s12934-022-01880-8. Free PMC article

・Toya Y, Hirono-Hara Y, Hirayama H, Kamata K, Tanaka R, Sano M, Kitamura S, Otsuka K, Abe-Yoshizumi R, Tsunoda SP, Kikukawa H, Kandori H, Shimizu H, Matsuda F, Ishii J, Hara KY: Optogenetic reprogramming of carbon metabolism using light-powering microbial proton pump systems. Metab Eng. 2022 Mar 26;72:227-236. doi:10.1016/j.ymben.2022.03.012. PubMed PMID:31799427; PubMed Central PMCID: PMC35346842 詳しい研究内容は、静岡県立大学 プレスリリース

・Hirono-Hara Y, Mizutani Y, Murofushi K, Iwahara K, Sakuragawa S, Kikukawa H, Hara KY：Effect of spent coffee grounds extract on astaxanthin production by Xanthophyllomyces dendrorhous. Bioresorce Technology Reports. 2022 Feburary;17:100953. doi: 10.1016/j.biteb.2022.100953. Free PMC article

・Kikukawa H, Shimizu C, Hirono-Hara Y, Hara KY：Effect of ethanol on astaxanthin and fatty acid production in the red yeast Xanthophyllomyces dendrorhous. Journal of Applied Microbiology. 2022 Mar; 132:2034-2041. doi: 10.1111/jam.15335.

・Hara KY, Yagi S, Hirono-Hara Y, Kikukawa H：A method of solubilizing and concentrating astaxanthin and other carotenoids. Marine Drugs. 2021, 19: 462. doi.org/10.3390/md19080462. Free PMC article

・Hirono-Hara Y, Mizutani Y, Murofushi K, Iwahara K, Sakuragawa S, Kikukawa H, Hara KY：Glutathione fermentation by Millerozyma farinosa using spent coffee grounds extract and seawater. Bioresorce Technology Reports. 2021 September;15:100777. doi: 10.1016/j.biteb.2021.100777. Free PMC article

・Kikukawa H, Shimizu C, Hirono-Hara Y, Hara KY：Screening of plant oils promoting growth of the red yeast Xanthophyllomyces dendrorhous with astaxanthin and fatty acid production. Biocatalysis and Agricultural Biotechnology. 2021 August;35:102101. doi: 10.1016/j.bcab.2021.102101.

・Kikukawa H, Okaya T, Maoka T, Miyazaki M, Murofushi K, Kato T, Hirono-Hara Y, Katsumata M, Miyahara S, Hara KY：Carotenoid nostoxanthin production by Sphingomonas sp. SG73 isolated from deep sea sediment. Marine Drugs. 2021 May 14;19(5):274. doi: 10.3390/md19050274. Free PMC article

・Hirono-Hara Y, Yui M, Hara KY：Production of transglutaminase in glutathione-producing recombinant Saccharomyces cerevisiae, AMB Express. 2021 Jan 07;11:13 doi: 10.1186/s13568-020-01176-3. Free PMC article

・Hara KY, Kageyama Y, Tanzawa N, Hirono-Hara Y, Kikukawa H, Wakabayashi K：Development of astaxanthin production from citrus peel extract using Xanthophyllomyces dendrorhous. Environ. Sci. Pollut. Res. 2020 Oct 21; 28:12640-12647. doi: 10.1007/s11356-020-11163-7. Free PMC article

・Imai Y, Inoshita T, Meng H, Shiba-Fukushima K, Hara KY, Sawamura N, Hattori N. Light-driven activation of mitochondrial proton-motive force improves motor behaviors in a Drosophila model of Parkinson's disease. Commun Biol. 2019 Nov 22;2:424. doi: 10.1038/s42003-019-0674-1. eCollection 2019. PubMed PMID:31799427; PubMed Central PMCID: PMC6874642 <DOI: 10.1038/s42003-019-0674-1>詳しい研究内容は、順天堂大学 プレスリリース

・Hara KY, Saito M, Kato H, Morikawa K, Kikukawa H, Nomura H, Fujimoto T, Hirono-Hara Y, Watanabe S, Kanamaru K, Kondo A. 5-Aminolevulinic acid fermentation using engineered Saccharomyces cerevisiae. Microb Cell Fact. 2019 Nov 7;18(1):194. doi: 10.1186/s12934-019-1242-6. PubMed PMID: 31699086. Free PMC article

・Kawaguchi H, Yoshihara K, Hara KY, Hasunuma T, Ogino C, Kondo A. Metabolome analysis-based design and engineering of a metabolic pathway in Corynebacterium glutamicum to match rates of simultaneous utilization of D-glucose and L-arabinose. Microb Cell Fact. 2018 May 17;17(1):76.Free PMC article

・Prima A, Hara KY, Djohan AC, Kashiwagi N, Kahar P, Ishii J, Nakayama H,Okazaki F, Prasetya B, Kondo A, Yopi, Ogino C. Glutathione production from　mannan-based bioresource by mannanase/mannosidase expressing Saccharomyces cerevisiae. Bioresource Technolgy 2017 Dec;245(Pt B):1400-1406.

・Kobayashi J, Sasaki D, Hara KY, Hasunuma T, Kondo A. (2017) Enzymatic improvement of mitochondrial thiol oxidase Erv1 for oxidized glutathione fermentation by Saccharomyces cerevisiae. Microb Cell Fact,16(1):44. Free PMC article

・Yamamoto K, Hara KY, Morita T, Nishimura A, Sasaki D, Ishii J, Ogino C, Kizaki N, Kondo A. (2016) Enhancement of astaxanthin production in Xanthophyllomyces dendrorhous by efficient method for the complete deletion of genes. Microb Cell Fact. 15(1):155. Free PMC article

・Ishii J, Okazaki F, Djohan AC, Hara KY, Asai-Nakashima N, Teramura H, Andriani A, Tominaga M, Wakai S, Kahar P, Yopi, Prasetya B, Ogino C, Kondo A. (2016) From mannan to bioethanol: cell surface co-display of β-mannanase and β-mannosidase on yeast Saccharomyces cerevisiae. Biotechnol Biofuels. 9(1):188. Free PMC article

・Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A. (2016)Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems. Science. 353(6305).<DOI:10.1126/science.aaf8729>プレスリリースは、こちら

・Sasaki K, Hara KY, Kawaguchi H, Sazuka T, Ogino C, Kondo A. (2016) Nanofiltration concentration of extracellular glutathione produced by engineered Saccharomyces cerevisiae. J Biosci Bioeng. 121(1):96-100.

・Hara KY, Aoki N, Kobayashi J, Kiriyama K, Nishida K, Araki M, Kondo A. (2015) Improvement of oxidized glutathione fermentation by thiol redox metabolism engineering in Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 99(22):9771-9778.

・Inokuma K, Ishii J, Hara KY, Mochizuki M, Hasunuma T, Kondo A. (2015) Complete Genome Sequence of Kluyveromyces marxianus NBRC1777, a Nonconventional Thermotolerant Yeast. Genome Announc. 3(2), e00389-15Free PMC article

・Ye X, Morikawa K, Ho SH, Araki M, Nishida K, Hasunuma T, Hara KY (Corresponding author), Kondo A. (2015) Evaluation of genes involved in oxidative phosphorylation in yeast by developing a simple and rapid method to measure mitochondrial ATP synthetic activity. Microb Cell Fact 14, 56. Free PMC article

・Hideo Kawaguchi, Hiroshi Teramura, Kouji Uematsu, Kiyotaka Y. Hara, Tomohisa Hasunuma, Ko Hirano, Takashi Sazuka, Hidemi Kitano, Yota Tsuge, Prihardi Kahar, Satoko Niimi-Nakamura, Ken-Ichi Oinuma, Naoki Takaya, Shigemitsu Kasuga, Chiaki Ogino, and Akihiko Kondo (2015) Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse. Bioresource Technology, 182, 169-178.

・Kiyotaka Y. Hara, Toshihiko Morita, Masao Mochizuki, Keisuke Yamamoto, Chiaki Ogino, Michihiro Araki, Akihiko Kondo (2014) Development of a multi-gene expression system in Xanthophyllomyces dendrorhous. Microbial Cell Factories 13(1), 175. Free PMC article

・Michihiro Araki, Robert Sidney Cox III, Hiroki Makiguchi, Teppei Ogawa, Takeshi Taniguchi, Kohei Miyaoku, Masahiko Nakatsui, Kiyotaka Y. Hara, Akihiko Kondo (2014) M-path: a compass for navigating potential metabolic pathways. Bioinformatics. 31(6), 905-911.

・Kiyotaka Y. Hara, Toshihiko Morita, Yusuke Endo, Masao Mochizuki, Michihiro Araki, Akihiko Kondo (2014) Evaluation and screening of efficient promoters to improve astaxanthin production in Xanthophyllomyces dendrorhous. Applied Microbiology and Biotechnology 98(15), 6787-6793.

・Shih-Hsin Ho, Akihito Nakanishi, Xiaoting Ye, Jo-Shu Chang, Kiyotaka Hara, Tomohisa Hasunuma, Akihiko Kondo (2014) Optimizing biodiesel production in marine Chlamydomonas sp.JSC4 through metabolic profiling and an innovative salinity-gradient strategy. Biotechnology for Biofuel 7, 97. Free PMC article

・Kentaro Kiriyama, Kiyotaka Y. Hara (equal contribution), Akihiko Kondo (2013) Oxidized glutathione fermentation using Saccharomyces cerevisiae engineered for glutathione metabolism. Applied Microbiology and Biotechnology 97(16),7399-7404.

・Kiyotaka Y. Hara, Takeyoshi Wada, Kuniki Kino, Toru Asahi, Naoya Sawamura (2013) Construction of photoenergetic mitochondria in cultured mammalian cells.Scientific Reports 3, 1635. Free PMC article日本語概要は、こちらWebニュースは、こちら

・Kentaro Kiriyama, Kiyotaka Y. Hara (equal contribution), Akihiko Kondo (2012) Extracellular glutathione fermentation using engineered Saccharomyces cerevisiae expressing novel glutathione exporter. Applied Microbiology and Biotechnology 96(4),1021-1027.

・Jun Ishii, Miyuki Moriguchi, Kiyotaka Y. Hara, Seiji Shibasaki, Hideki Fukuda, Akihiko Kondo (2012) Improved identification of agonist-mediated Gαi-specific human G-protein-coupled receptor signaling in yeast cells by flow cytometry. Analytical Biochemistry 426(2), 129-133.

・Kiyotaka Y. Hara, Songhee Kim, Kentaro Kiriyama, Hideyo Yoshida, Syogo Arai, Jun Ishii, Chiaki Ogino, Hideo Fukuda, Akihiko Kondo (2012) An energy-saving glutathione production method from low-temperature cooked rice using amylase-expressing Saccharomyces cerevisiae. Biotechnology Journal 7(5),686-689.

・Kiyotaka Y. Hara, Kentaro Kiriyama, Hideo Nakayama, Akihiko Kondo (2012) Improvement　of glutathione production by metabolic engineering the sulfate assimilation pathway of Saccharomyces cerevisiae. Applied Microbiology and Biotechnology 94(5),1313-1319.

・Kiyotaka Y. Hara, Songhee Kim, Hideyo Yoshida, Kentaro Kiriyama, Takashi Kondo, Naoko Okai, N, Chiaki Ogino, Hideo Fukuda, Akihiko Kondo (2012) Development of a glutathione production process from proteinaceous biomass resources using protease-displaying Saccharomyces cerevisiae. Applied Microbiology and Biotechnology 93(4),1495-1502.

・Hideyo Yoshida, Kiyotaka Y. Hara (equal contribution), Kentro Kiriyama, Hideo Nakayama, Fumio Matsuda,Chiaki Ogino, Hideo Fukuda, Akihiko Kondo (2011) Enzymatic glutathione production using metabolically engineered Saccharomyces cerevisiae as a whole-cell biocatalyst., Applied Microbiology and Biotechnology 91(4),1001-1006.

・Kiyotaka Y. Hara, Rie Suzuki, Toshiharu Suzuki, Masasuke Yoshida, Kuniki Kino (2011) ATP photosynthetic vesicles for light-driven bioprocesses. Biotechnology Letters 33(6),1133-1138.

・Hideyo Yoshida, Shogo Arai, Kiyotaka Y. Hara, Ryosuke Yamada, Chiaki Ogino, Hideo Fukuda, Akihiko Kondo (2011) Efficient and direct glutathione production from raw starch using Saccharomyces cerevisiae. Applied Microbiology and Biotechnology 89(5),1417-1422.

・Kiyotaka Y. Hara, Natsuka Shimodate, Yasutaka Hirokawa, Mikito Ito, Tomoya Baba, Hirotada Mori, Hideo Mori (2009) Glutathione production by efficient ATP-regenerating Escherichia coli mutants. FEMS Microbiology Letters 297(2), 217-214.

・Kiyotaka Y. Hara, Natsuka Shimodate, Mikito Ito, Tomoya Baba, Hirotada Mori, Hideo Mori (2009) Systematic genome-wide scanning for genes involved in ATP generation in Escherichia coli. Metabolic Engineering 11(1),1-7.

・Kiyotaka Y. Hara, Hideo Mori (2006) An efficient method for quantitative determination of cellular ATP synthetic activity. Journal of Biomolecular Screening 11(3),310-317.

・Katsuya Shimabukuro, Ryohei Yasuda, Eiro Muneyuki, Kiyotaka Y. Hara, Kazuhiko Kinosita, Jr, Masasuke Yoshida (2003) Catalysis and rotation of F1 motor: cleavage of ATP at the catalytic site occurs in 1 ms before 40 degree substep rotation. Proceedings of National Academy of Sciences of the United States of America 100(25),14731-14736. Free PMC article

・Sakurako Ono, Kiyotaka Y. Hara, Jun Hirao, Tadashi Matsui, Hiroyuki Noji, Masasuke Yoshida, Eiro Muneyuki (2003) Origin of apparent negative cooperativity of F(1)-ATPase., Biochimica et Biophysica Acta -Bioenergetics 1607(1),35-44. Free article

・Yoko Hirono-H, Hiroyuki Noji, Masaya Nishiura, Eiro Muneyuki, Kiyotaka Y. Hara, Ryohei Yasuda, Kazuhiko Kinosita, Jr, Masasuke Yoshida (2001) Pause and rotation of F(1)-ATPase during catalysis. Proceedings of National Academy of Sciences of the United States of America 98(24),13649-13654. Free PMC article

・Kiyotaka Y. Hara, Yasuyuki Kato-Yamada, Yuji Kikuchi, Toru Hisabori, Masasuke Yoshida (2001) The role of the betaDELSEED motif of F1-ATPase: propagation of the inhibitory effect of the epsilon subunit. Journal of Biological Chemistry 276(26), 23969-23973. Free article

・Kiyotaka Y. Hara, Hiroyuki Noji, Dirk Bald, Ryohei Yasuda, Kazuhiko Kinosita, Jr., Masasuke Yoshida (2000) The role of the DELSEED motif of the beta subunit in rotation of F1-ATPase. Journal of Biological Chemistry 275(19), 14260-14263. Free article

・Toyoki Amano, Tadashi Matsui, Eiro Muneyuki, Hiroyuki Noji, Kiyotaka Hara, Masasuke Yoshida, Toru Hisabori (1999) alpha3beta3gamma complex of F1-ATPase from thermophilic Bacillus PS3 can maintain steady-state ATP hydrolysis activity depending on the number of non-catalytic sites. Biochemical Journal 343(1), 135-138. Free PMC article